School of Chemistry

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Blue Bottle Experiment

Description

In the demonstration, a bottle of colourless liquid is shaken for a few seconds until the liquid turns blue. Upon standing for about a minute, the solution goes clear again. This sequence can be repeated many times.


How does it happen?

Essentially, it is an oxidation-reduction reaction. The bottle contains potassium hydroxide, glucose, water, and an indicator called "methylene blue". Glucose is a reducing agent and in alkaline solution it will reduce methylene blue to a colourless form. Shaking the bottle allows the oxygen in the air to oxidise the methylene blue to the blue form. Upon standing it is reduced by the glucose back to the colourless form. The equations for the reaction are:

when shaken:O2 + MB (colourless) -> MBox (blue)
upon standing:RH + OH- -> R- + H2O
MBox + R- -> MB + oxidation products of glucose

where MB is the reduced (colourless) form of methylene blue, MBox is the oxidised (blue) form of methylene blue, and RH represents glucose.

Methylene blue is an example of a "redox indicator". Some other redox indicators you may come across are ferroin, diphenylamine and indigo carmine.


Further details

This demonstration was taken from Ted Lister's Classic Chemistry Demonstrations, published by the Royal Society of Chemistry, pages 48-49. Further details of the reaction mechanism are given in AG Cook, RM Tolliver, JE Williams J. Chem. Ed. 1994, 71, 160-161. Another article of interest is that by WR Vandaveer, M Mosher J. Chem. Ed. 1997, 74, 402.


This page was devised by Dr Brian Yates, a lecturer in the School of Chemistry. Please contact me if you have any questions. E-mail: Brian.Yates@utas.edu.au